Mouse anti Sheep GM-CSF: 3C2

Description:

Mouse anti Sheep GM-CSF, clone 3C2 recognizes both recombinant and native ovine Granulocyte-macrophage colony-stimulating factor, also known GM-CSF or CSF, a 16.3kDa cytokine responsible for the stimulation and growth of cells from granulocyte, macrophage, eosinophil and erythrocyte hematopoietic precursor cell linages.

Mouse anti sheep GM-CSF, clone 3C2 has is of particular interest as it has been shown to reconize GM-CSF in cattle while other clones such as 8D8 do not crosss react with cattle.
Mouse anti sheep GM-CSF, clone 3C2 has been reported to neutralise the activity of both recombinant ovine and native GM-CSF.
Removal of sodium azide is recommended prior to use in functional assays.

Product Form:

Purified IgG - liquid.

Preparation:

Purified IgG prepared by affinity chromatography on protein G from tissue culture supernatant.

Buffer :

Phosphate buffered saline.

Preservative Stabilisers:

0.09% Sodium azide.

Carrier Free:

Yes.

Immunogen:

CHO expressed recombinant ovine GM-CSF.

Approx. Protein Concentrations:

IgG concentration 1.0 mg/ml.

Fusion Partners:

Spleen cells from immunised Balb/c mice were fused with cells of the NSO myeloma cell line.

Regulatory:

For research purposes only.

Guarantee:

12 months from date of despatch.

Application:

ELISA, western blotting.

Storage:

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Note:

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.