Mouse anti Sheep Interleukin-8: 2C9

Description:

Mouse anti sheep interleukin-8 antibody, clone 2C9, recognizes ovine interleukin-8 (IL-8), also known as C-X-C motif chemokine 8. IL-8 is a 101 amino acid ~16 kDa chemoattractant for neutrophils, basophils and T-cells. IL-8 is produced by several cell types including neutrophils, monocytes and macrophages in response to inflammatory stimulation. Mouse anti Sheep interleukin-8 antibody, clone 2C9 shows no cross-reactivity with ovine IL-1 beta, IL-6, MCP-1 or TNF alpha.

Responses to infectious stimuli may vary among ovine species, the response to Mannheimia haemolytica, a causative agent of pneumonia, peritonitis and gangrenous mastitis in ovids, is exaggerated in bighorn sheep (Ovis canadensis) compared to domestic sheep (Ovis aries) with significantly elevated IL-8 levels in response to infection.

Mouse anti sheep interleukin-8 antibody, clone 2C9, has been shown to compete with clone 8M6 for binding of recombinant ovine IL-8 by ELISA, suggesting that the two clones recognize the same or a closely-related epitope.

Product Form:

Purified IgG - liquid.

Preparation:

Purified IgG prepared by affinity chromatography on protein A from tissue culture supernatant.

Buffer :

Phosphate buffered saline.

Preservative Stabilisers:

0.09% Sodium azide (NaN₃).

Carrier Free:

Yes.

Immunogen:

Recombinant sheep IL-8.

Approx. Protein Concentrations:

IgG concentration 1.0 mg/ml.

Fusion Partners:

Spleen cells from immunised Balb/c mice were fused with cells of the NSO myeloma cell line.

Regulatory:

For research purposes only.

Guarantee:

12 months from date of despatch.

Application:

ELISA.

Storage:

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Note:

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.