Mouse anti Sheep Interleukin-8

Description:

Mouse anti ovine interleukin-8 antibody, clone 8M6 recognizes ovine interleukin-8 (IL-8), also known as C-X-C motif chemokine 8. IL-8 is a 79 amino acid ~9-11 kDa chemoattractant for neutrophils, basophils and T-cells. IL-8 is produced by several cell types including neutrophils, monocytes and macrophages in response to inflammatory stimulation. Mouse anti ovine interleukin-8 antibody, clone 8M6 shows no cross-reactivity with ovine IL-1 beta, IL-6, MCP-1 or TNF alpha.

Responses to infectious stimuli may vary among ovine species, the response to Mannheimia haemolytica, a causative agent of pneumonia, peritonitis and gangrenous mastitis in ovids, is exaggerated in Bighorn sheep compared to domestic sheep with significantly elevated IL-8 levels in response to infection.

Mouse anti ovine interleukin-8 antibody, clone 8M6 has been utilized to identify cells and cell types expressing IL-8 in inflamed porcine tissue showing here also that neutrophils are the predominant cell type expressing IL-8 whilst epithelial and endothelial cells in the vicinity of inflammatory lesions also express the cytokine. Clone 8M6 neutralizes the bioactivity of ovine IL-8.
Mouse anti ovine interleukin-8 antibody, clone 8M6 has been used in conjunction with rabbit anti sheep Interleukin-8 antibody for the development of a sensitive ELISA to measure IL-8 concentrations in bovine samples.

Product Form:

Purified IgG - liquid.

Buffer :

Phosphate buffered saline.

Preservative Stabilisers:

<0.1% Sodium azide (NaN₃).

Immunogen:

Recombinant bovine IL-8.

Approx. Protein Concentrations:

IgG concentration 1.0 mg/ml.

Regulatory:

For research purposes only.

Guarantee:

12 months from date of despatch.

Application:

ELISA, flow cytometry, functional assays, Western blotting.

Storage:

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Note:

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.