Mouse anti Sheep MHC Class I

Description:

Mouse anti sheep MHC Class I antibody, clone VPM19 recognizes the ovine homologue of the human MHC Class I, a monomorphic determinant expressed on the heavy chain of sheep MHC Class I, (OLA Class I).

The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In sheep, this is often referred to as the ovine leukocyte antigen (OLA) region. Ovine MHC Class I functions in the recognition and presentation of foreign antigens to T-cells.

Ovine MHC Class I is a membrane glycoprotein with a molecular weight of approximately 44kDa, expressed on the cell surface of all peripheral blood leucocytes.

Clone VPM19 has been used in a number of domestic animal disease states, in particular Maedi Visna virus infection, a disease of significant importance in commercial sheep flocks. Mouse anti sheep MHC Class I antibody, clone VPM19 recognizes MHC class I in other species and has been used in a study of feline herpes virus infection.

Product Form:

Purified IgG - liquid.

Preparation:

Purified IgG prepared by affinity chromatography on protein A from tissue culture supernatant.

Buffer :

Phosphate buffered saline.

Preservative Stabilisers:

0.09% Sodium azide (NaN₃).

Carrier Free:

Yes

Immunogen:

Sheep T cells.

Approx. Protein Concentrations:

IgG concentration 1.0 mg/ml.

Fusion Partners:

Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS0 myeloma cell line.

Regulatory:

For research purposes only.

Guarantee:

12 months from date of despatch.

Application:

Flow cytometry, immunohistology - frozen, immunoprecipitation, Western blotting.

Storage:

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Note:

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.