Mouse Haptoglobin ELISA Assay

Product Overview:

The mouse haptoglobin ELISA assay kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring haptoglobin in serum or plasma of mice.

Product Description:

Acute phase proteins are plasma proteins which increase in concentration following infection, inflammation or trauma. The first acute phase protein to be recognized was discovered in humans by Tillet and Frances in 1930. Haptoglobin (Hp) is a heterogeneous plasma protein mostly synthesized in by the liver. The haptoglobin monomer consist of two heavy chains, beta chains (40 kD) and two light chains, alpha chains, alpha 1 (9 kD) and alpha 2 (16 kD) that are linked disulfide bonds. The three major haptoglobin types are; Hp1-1 which is monomeric (98kD), Hp1-2 is polymeric at about 200 kD, and Hp2-2 at about 400 kD. The haptoglobin level in serum rise quickly following acute tissue damage within 24 to 48 hours and also falls very rapidly once the stimulus is removed. In fact, Haptoglobin levels are decreased in hemolytic anemia. Hp has a high affinity for hemoglobin (Hb) and its function appears to be to prevent loss of Hb in urine which would lead to loss of iron. Investigations over the past few years have shown that quantification of Hp in plasma or serum can provide valuable diagnostic information in the detection, prognosis, and monitoring of disease not only in humans, but in companion animals and farm herds as well.

Sensitivity:

0.586 ng/ml

Dynamic Range:

1.95 ng/ml – 125 ng/ml

Incubation Time:

40 minutes

Sample Type:

Plasma, serum

Sample Size:

100 µl