Mouse IL-10 ELISA Assay

Product Overview:

The mouse interleukin 10 (IL-10) ELISA assay kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of mouse Interleukin 10 (IL-10) concentrations in cell culture supernates, serum, and plasma.

Assay Principle:

The mouse interleukin 10 (IL-10) ELISA assay kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-10 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IL-10 is added to the wells and binds to the combination of capture antibody-IL-10 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of IL-10 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-10 standard dilutions and IL-10 sample concentration determined.

Sensitivity:

7 pg/ml

Dynamic Range:

31.25 – 2000 pg/ml

Incubation Time:

3.5 hours

Sample Type:

Cell culture, serum, plasma

Sample Size:

100 µl