Mouse IL-12 ELISA Assay

Product Overview:

Mouse IL-12 ELISA assay kit is used as an analytical tool for quantitative determination of mouse interleukin 12, IL-12/P70 in serum and plasma. The mouse IL-12 ELISA assay kit is for research use only and not to be used for diagnostic procedures.

Product Description:

The Mouse IL-12/P70 ELISA Assay Kit is used for assessing the specific biomarker in samples analytes which may be serum / plasma as validated with the kit. The kit employs a sandwhich ELISA technique which leads to a higher specificity and increased sensitivity compared to conventional competitive ELISA kits which employ only one antibodies. Double antibodies are used in this kit.

Assay Principle:

The method employs sandwhich ELISA technique. Monoclonal antibodies are pre-coated onto microwells. Samples and standards are pipetted into microwells and Mouse Interleukin 12, IL-12/P70 present in the sample are bound by the antibodies. Then, a HRP (horseradish peroxidase) conjugated secondary antibody is pipetted and incubated to form a complex. After washing microwells in order to remove any non-specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Mouse Interleukin 12, IL-12/P70 in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.

Sensitivity:

4.52 ng/ml

Dynamic Range:

10 – 2000 ng/ml

Incubation Time:

3.5 hours

Sample Type:

Plasma, serum

Sample Size:

100 µl