Mouse IL-12p70 ELISA Assay
Products Name
Mouse IL-12p70 ELISA Assay
Cat.No.
IVEK0242
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Overview
Product Overview:
Mouse interleukin 12 p70 (IL-12 p70) ELISA assay kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of mouse Interleukin 12 p70 (IL-12 p70) concentrations in cell culture supernates, serum, and plasma.
Assay Principle:
The mouse interleukin 12 p70 (IL-12 p70) ELISA assay kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-12 p70 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-12 p70 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IL-12 p70 is added to the wells and binds to the combination of capture antibody- IL-12 p70 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of IL-12 p70 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-12 p70 standard dilutions and IL-12 p70 sample concentration determined.
Sensitivity:
7 pg/ml
Dynamic Range:
31.25 – 2000 pg/ml
Incubation Time:
3.5 hours
Sample Type:
Cell culture, serum, plasma
Sample Size:
100 µl
Specimen Collection and Handling:
Cell culture supernates – remove particulates by centrifugation.
Serum – use a serum separator tube (SST) and allow samples to clot for 30 minutes before centrifugation for 15 minutes at approximately 1000 x g. Remove serum, avoid hemolysis and high blood lipid samples.
Plasma – recommended EDTA as an anticoagulant in plasma. Centrifuge for 15 minutes at 1000 x g within 30 minutes of collection.
Assay immediately or aliquot and store samples at -20°C. Avoid repeated freeze-thaw cycles.
Dilute samples at the appropriate multiple (recommended to do pre-test to determine the dilution factor).
Serum – use a serum separator tube (SST) and allow samples to clot for 30 minutes before centrifugation for 15 minutes at approximately 1000 x g. Remove serum, avoid hemolysis and high blood lipid samples.
Plasma – recommended EDTA as an anticoagulant in plasma. Centrifuge for 15 minutes at 1000 x g within 30 minutes of collection.
Assay immediately or aliquot and store samples at -20°C. Avoid repeated freeze-thaw cycles.
Dilute samples at the appropriate multiple (recommended to do pre-test to determine the dilution factor).
