Mouse IL-4 ELISA Assay

Product Overview:

Mouse IL-4 ELISA assay kit is used as an analytical tool for quantitative determination of mouse interleukin 4, IL-4, in serum, plasma and other biological samples.

Product Description:

The Mouse IL-4 ELISA Assay Kit is used for assessing the specific biomarker in samples analytes which may be serum, plasma and cell culture supernatant as validated with the kit. The kit employs a sandwich ELISA technique which leads to a higher specificity and increased sensitivity compared to conventional competitive ELISA kits which employ only one antibody. Double antibodies are used in this kit.

Assay Principle:

The method employs sandwich ELISA technique. Monoclonal antibodies are pre-coated onto microwells. Samples and standards are pipetted into microwells and Mouse Interleukin 4, IL-4 present in the sample are bound by the antibodies. Biotin labeled antibody is added and followed by Streptavidin-HRP is pipetted and incubated to form a complex. After washing microwells in order to remove any non-specific binding, the substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Mouse Interleukin 4, IL-4 in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.

Sensitivity:

0.23 ng/ml

Dynamic Range:

0.5 – 200 ng/ml

Incubation Time:

1 hour 10 minutes

Sample Type:

Serum, plasma, or other biological samples

Sample Size:

40 µl