Mouse / Rat Adrenaline (Epinephrine) ELISA

Product Overview:

The mouse/rat adrenaline (epinephrine) ELISA is an enzyme-linked immunosorbent assay used for the quantitative and very sensitive determination of adrenaline in biological samples including serum, plasma, tissue, and cell culture samples.

Assay Principle:

The mouse / rat adrenaline (epinephrine) ELISA assay kit is an enzyme-linked immunosorbent assay used for the quantitative and very sensitive determination of adrenaline in mouse and rat biological samples including serum, plasma, tissue, and cell culture samples. The mouse / rat adrenaline (epinephrine) ELISA assay kit is for research use only and should not be used in diagnostic procedures. The competitive mouse / rat adrenaline ELISA assay kit uses the microtitre plate format. Adrenaline is bound to the solid phase of the microtiter plate. Acylated catecholamine from the sample and solid phase bound catecholamine compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase catecholamine is detected by anti-rabbit IgG/peroxidase. The substrate TMB/peroxidase reaction is monitored at 450 nm. The amount of antibody bound to the solid phase catecholamine is inversely proportional to the catecholamine concentration of the sample.

Sensitivity:

1.6 pg/ml

Dynamic Range:

0.15 – 25 ng/ml

Incubation Time:

Overnight

Sample Type:

Biological samples

Sample Size:

20 µl