Mouse Rat GLP-1 Active (7-36) ELISA
Products Name
Mouse Rat GLP-1 Active (7-36) ELISA
Cat.No.
IVEK0260
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Overview
Product Overview:
The mouse/rat active GLP-1 ELISA assay kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of bioactive glucagon-like peptide-1 (7-36) amide level in rat and mouse plasma samples with only 20 µl of sample volume.
Assay Principle:
The mouse / rat active GLP-1 ELISA assay kit is designed, developed and produced for the quantitative measurement of bioactive GLP-1 (7-36) in rodent plasma where there is usually have limited amount of sample available for analysis. The assay utilizes the two-site “sandwich” technique with two selected GLP-1 (7-36) specific antibodies.
The mouse / rat GLP-1 Active (7-36) ELISA has standards, controls and test samples that are directly added to wells of a microplate that is coated with streptavidin. Subsequently, a mixture of biotinylated GLP-1 (7-36) specific antibody and a horseradish peroxidase (HRP) conjugated GLP-1 (7-36) specific antibody is added to each well. After the first incubation period, a “sandwich” immunocomplex of “streptavidin – biotin-antibody – GLP-1(7-36) – HRP conjugated antibody” is formed and attached to the wall of the plate. The unbound HRP conjugated antibody is removed in a subsequent washing step. For the detection of this immunocomplex, each well is then incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the immunocomplex bound to GLP-1 (7-36) on the wall of the microtiter well is directly proportional to the amount of GLP-1 (7-36) in the sample.
The mouse / rat GLP-1 Active (7-36) ELISA has standards, controls and test samples that are directly added to wells of a microplate that is coated with streptavidin. Subsequently, a mixture of biotinylated GLP-1 (7-36) specific antibody and a horseradish peroxidase (HRP) conjugated GLP-1 (7-36) specific antibody is added to each well. After the first incubation period, a “sandwich” immunocomplex of “streptavidin – biotin-antibody – GLP-1(7-36) – HRP conjugated antibody” is formed and attached to the wall of the plate. The unbound HRP conjugated antibody is removed in a subsequent washing step. For the detection of this immunocomplex, each well is then incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the immunocomplex bound to GLP-1 (7-36) on the wall of the microtiter well is directly proportional to the amount of GLP-1 (7-36) in the sample.
Sensitivity:
0.1 pM
Dynamic Range:
0.85 – 146 pM
Incubation Time:
Overnight
Sample Type:
Plasma
Sample Size:
20 µl
