Mouse sRANK Ligand ELISA Assay

Product Overview:

The mouse sRANK ligand ELISA assay kit is intended or the quantification of mouse soluble receptor activator of NFkB ligand in serum, plasma, cell culture supernatants.

Assay Principle:

The mouse sRANK ligand ELISA assay employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for sRANK ligand / TNFSF11 / TRANCE has been pre-coated onto a microtiter plate. Standards or samples are pipetted into the wells and any sRANK ligand / TNFSF11 / TRANCE present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-conjugated antibody specific for sRANK Ligand / TNFSF11 / TRANCE is added to each well and incubate. Following a washing to remove unbound substances, streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After washing away any unbound antibody-enzyme reagent, a substrate solution (TMB) is added to the wells and color develops in proportion to the amount of sRANK Ligand / TNFSF11 / TRANCE bound in the initial step. The color development is stopped by the addition of acid and the intensity of the color is measured at a wavelength of 450nm ±2nm. The concentration of sRANK Ligand / TNFSF11 / TRANCE in the sample is then determined by comparing the O.D of samples to the standard curve.

Sensitivity:

15 pg/ml

Dynamic Range:

31.25-2000 µg/ml

Incubation Time:

4 hours

Sample Type:

Mouse serum, plasma, cell culture supernatants

Sample Size:

100 µl