Mouse TGF beta1 ELISA

Product Overview:

The mouse TGF beta1 ELISA assay kit is intended for the quantification of mouse transforming growth factor- beta 1 in serum, plasma, cell culture supernatants.

Assay Principle:

This TGF beta1 ELISA for mouse employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for TGF beta1 has been pre-coated onto a microtiter plate. Standards or samples are pipetted into the wells and any TGF beta1 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-conjugated antibody specific for TGF beta1 is added to each well and incubate. Following a washing to remove unbound substances, streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After washing away any unbound antibody-enzyme reagent, a substrate solution (TMB) is added to the wells and color develops in proportion to the amount of TGF beta1 bound in the initial step. The color development is stopped by the addition of acid and the intensity of the color is measured at a wavelength of 450nm ±2nm. The concentration of TGF beta1 in the sample is then determined by comparing the O.D of samples to the standard curve.

Sensitivity:

5 pg/ml

Dynamic Range:

31.25 pg/ml-2000 pg/ml

Incubation Time:

4 hours

Sample Type:

Cell culture supernates, serum and plasma

Sample Size:

100 µl