Mouse Total Legumain ELISA Kit

Product Overview:

The mouse total legumain ELISA kit is intended for the quantitation of mouse LGMN concentrations in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

Product Description:

The Mouse Lgmn Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Mouse Lgmn with a 96-well strip plate that is pre-coated with antibody specific for Lgmn. The detection antibody is a biotinylated antibody specific for Lgmn. The capture antibody is monoclonal antibody from rat, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Mouse Lgmn with immunogen: Expression system for standard: NSO; Immunogen sequence: V18-Y435. The kit is analytically validated with ready to use reagents. To measure Mouse Lgmn, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Mouse Lgmn in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells’ readings against the standard curve to determine the concentration of Mouse Lgmn in the sample.

Sensitivity:

< 10 pg/ml

Dynamic Range:

31.2 pg/ml-2000 pg/ml

Incubation Time:

2 hours 40 minutes

Sample Type:

Cell culture supernates, cell lysates, serum and plasma (heparin, EDTA)

Sample Size:

100 µl