Mouse tPA Active ELISA Assay

Product Overview:

The mouse tPA active ELISA assay is for the quantitative determination of active tPA in mouse biological fluids by a ELISA assay (enzyme-linked immunosorbent assay).

Product Description:

Tissue-type Plasminogen Activator (tPA) is a member of the serine proteinase family. tPA functions to lyse fibrin clots into soluble plasmin fragments. tPA is active in two forms, single chain and two-chain. The two-chain tPA is created via interaction with the plasmin product cleaving the single chain. This two-chain form is regarded as the more active form. Both single chain and two-chain tPA are complexable with PAI-1. PAI-1 acts as an inhibitor for tPA by binding to the tPA and thus stifling its ability to lyse fibrin. tPA can serve as an indicator of both myocardial infarction for patients with impaired fibrinolytic systems as well as a marker for type-II diabetes.

Sensitivity:

0.1 ng/ml

Dynamic Range:

0.1 – 50 ng/ml

Incubation Time:

2.5 hours

Sample Type:

Biological fluids

Sample Size:

100 µl

Specimen Collection and Handling:

Samples should be collected using 0.1 M trisodium citrate or acidified citratein a 1:10 ratio of collection media to blood. Immediately upon collection of blood, the samples should be centrifuged at 3000 x g for 15 minutes. This should ensure the removal of platelets as they can release PAI-1, that in turn complexes with tPA. The plasma should be transferred to a clean plastic tube to be kept on ice prior to analysis or stored frozen for up to one month. Samples are stable for approximately 24 hours when stored at 4°C or one month if stored at -20°C and thawed three times without loss of tPA activity. Note: Detergents such as Triton X cause interference with the assay. If using detergent extracted samples, it is necessary to dialyze the samples overnight to remove the detergent.