Mouse/Rat Dopamine ELISA

Product Overview:

The mouse/rat dopamine ELISA assay kit is an enzyme-linked immunosorbent assay used for the quantitative and very sensitive determination of dopamine in biological samples including serum, plasma, tissue, and cell culture samples.

Assay Principle:

The mouse / rat dopamine assay kit provides materials for the quantitative measurement of dopamine in low concentrated samples and for small sample volumes. In the mouse / rat dopamine assay, dopamine is extracted using a cis-diol-specific affinity gel and acylated to N-acyldopamine and then converted enzymatically into N-acyl-3-methoxytyramine. The mouse / rat dopamine ELISA kit uses the microtiter plate format. Dopamine is bound to the solid phase of the microtiter plate. Acylated catecholamine from the sample and solid phase bound catecholamine compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase catecholamine is detected by anti-rabbit IgG / peroxidase. The substrate TMB / peroxidase reaction is monitored at 450 nm. The amount of antibody bound to the solid phase catecholamine is inversely proportional to the catecholamine concentration of the sample.

Sensitivity:

5.9 pg/ml

Dynamic Range:

0.3 – 100 pg/ml

Incubation Time:

Overnight

Sample Type:

Biological samples

Sample Size:

20 µl