Mouse/Rat GH ELISA Assay

Product Overview:

The mouse/rat GH ELISA assay kit is intended to be used for the measurement of growth hormone in mouse and rat serum and plasma samples.

Assay Principle:

The mouse/rat GH ELISA assay kit is a so-called sandwich-assay. It utilizes two different specific high affinity polyclonal antibodies for this protein. The GH in the samples binds quantitatively to the immobilized antibody. In the following step, the biotinylated antibody in turn binds GH. After washing, a streptavidin-peroxidase-enzyme conjugate will be added, which will bind highly specific to the biotin of the antibody. Subsequently, the peroxidase catalyzes an enzymatic reaction resulting in a blue coloration. The intensity of the blue color depends on the GH content of the sample. The reaction is stopped by the addition of stop solution and color intensity is quantified by measuring the absorption.

Sensitivity:

0.04 ng/ml

Incubation Time:

3 hours

Sample Type:

Plasma, serum

Sample Size:

20 µl

Specimen Collection and Handling:

It is recommended to store samples chilled as soon as possible.
For any longer time storage the sample has to be kept frozen at -20°C.