Description:
Native rabbit phosphorylase B is a member of the protein kinase superfamily. Phosphorylases catalyse the breakdown of glycogen in liver and muscle. The phosphorylase of skeletal muscle occurs in two forms, the active form (phosphorylase-a) and a much less active form (phosphorylase b).
Phosphorylase b has a molecular weight of approximately 185kDa.
Product Form:
Purified enzyme from rabbit skeletal muscle - liquid.
Buffer :
Phosphate buffered saline.
Preservative Stabilisers:
None present.
Activity:
55-60 U/mg protein.
One unit will liberate one µM of glucose-1-phosphate from glycogen and orthophosphate per minute at 37ºC and pH 6.8.
Approx. Protein Concentrations:
5.0 mg/ml.
Regulatory:
For research purposes only.
Guarantee:
12 months from date of despatch.
Storage:
Store at -20°C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Note:
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Product Type:
Purified protein
Specificity:
Phosphorylase B