Description:
Rabbit anti chicken interferon alpha antibody recognizes chicken Interferon alpha (chIFNα). Similarities exist between chicken IFN and mammalian IFNI, revealing the conservation of cysteine residues and the existence of around ten closely related intron-less IFN alpha genes, chIFN-alpha genes are strongly expressed following viral infection of monocyte-derived macrophage and embryo fibroblasts and acts as a potent antiviral agent.
Rabbit anti chicken interferon alpha antibody cross reacts with IFN beta in western blots but does not show any cross reactivity with IFN gamma and in bioassays, rabbit anti chicken interferon alpha antibody neutralizes chIFN-alpha, but not chIFN-beta, and can therefore be used to discriminate IFN alpha and IFN beta bioactivities.
Product Form:
Purified IgG - liquid.
Preparation:
Antisera to chicken Interferon alpha were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography on protein A.
Buffer :
Phosphate buffered saline.
Preservative Stabilisers:
None present.
Immunogen:
Recombinant chicken IFNα.
Approx. Protein Concentrations:
IgG concentration 0.5 mg/ml.
Regulatory:
For research purposes only.
Guarantee:
12 months from date of despatch.
Application:
ELISA, functional assays, immunoprecipitation, western blotting.
Storage:
Store at -20°C only.
Note:
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Product Type:
Polyclonal antibody