Rabbit anti Chicken Interferon Gamma:Preservative Free

Description:

Rabbit anti chicken interferon gamma antibody recognizes chicken interferon gamma (chIFN-gamma). There are at least three distinct chicken interferon proteins, designated as chIFN-alpha, chIFN-beta and chIFN-gamma.

chIFN-gamma was first identified, when supernatant from chicken spleen cells stimulated with concanavalin A (ConA ), displayed macrophage-activating factor (MAF) activity - the principle function of mammalian type II IFN-gamma. Cloning of IFN-gamma cDNA from activated chicken T cells indicates that recombinant chicken IFN-gamma activates macrophages, strongly upregulating the expression of MHC class II and iNOS genes.

Rabbit anti chicken interferon gamma antibody can be used to neutralize chIFN-gamma activity and to discriminate the antiviral activity of chIFN-gamma from that of chIFN-alpha and chIFN-beta.

Product Form:

Purified IgG - liquid.

Preparation:

Antisera to chicken Interferon Gamma were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography on protein A.

Buffer :

Phosphate buffered saline.

Preservative Stabilisers:

None present.

Immunogen:

Recombinant chicken interferon gamma.

Approx. Protein Concentrations:

IgG concentration 0.5 mg/ml.

Regulatory:

For research purposes only.

Guarantee:

12 months from date of despatch.

Application:

ELISA, functional assays, western blotting.

Storage:

Store at -20°C only.

Note:

Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.