Rabbit anti Chicken Interleukin-6:Preservative Free

Description:

Rabbit anti chicken interleukin-6 antibody recognizes chicken Interleukin 6 (chIL-6).

Mammalian IL-6 is a multifunctional pro-inflammatory cytokine, which plays a major role in the regulation of immune responses, haematopoiesis and the induction of acute phase responses. The action of IL-6 occurs via the signal transducing receptor chain gp130 and the IL-6 receptor alpha chain is responsible for ligand binding.

IL-6 is produced by a wide variety of cells including B lymphocytes, T lymphocytes, monocytes/macrophages, fibroblasts and endothelial cells and has a widespread cellular effect, including B and T lymphocytes, hepatocytes and cells of the CNS.

Chicken hepatocyte-stimulating factor (HSF), secreted by lipopolysaccharide (LPS)-stimulated macrophages, induces fibronectin production in cultured chicken hepatocytes, and that HSF produced by LPS-treated fibroblasts, was neutralized by an antiserum to recombinant HuIL-6 (rHuIL-6). rHuIL-6 has also been shown as biologically active on chicken hepatocytes.

Rabbit anti chicken interleukin-6 antibody neutralizes chicken IL-6 in the murine 7TD1 IL-6 bioassay.

Product Form:

Purified IgG - liquid.

Preparation:

Antisera to chicken Interleukin 6 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography on protein G.

Buffer :

Phosphate buffered saline.

Preservative Stabilisers:

None present.

Immunogen:

Recombinant chicken IL-6.

Approx. Protein Concentrations:

IgG concentration 0.5 mg/ml.

Regulatory:

For research purposes only.

Guarantee:

12 months from date of despatch.

Application:

ELISA, functional assays, western blotting.

Storage:

Store at -20°C only.

Note:

Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.