Rat anti Dog CD44

Description:

Rat anti dog CD44 antibody, clone YKIX337.8.7 recognizes canine CD44, also known as H-CAM, a single-pass type 1 membrane of approximately 90 kDa expressed by most leucocytes and epithelial cells. CD44 expression is markedly increased upon cell activation.

CD44 is involved in cell-cell, cell adhesion and cell migration and is the principal cellular receptor for hyaluronate via it's LINK domain, additionally CD44 interacts with other ligands including collagens and metalloproteinases.

Altered CD44 expression is detected in many forms of invasive and metastatic cancer, CD44 expression has been observed on canine mammary and melanocytic tumors.

Product Form:

Purified IgG - liquid.

Preparation:

Purified IgG prepared by affinity chromatography on protein G from tissue culture supernatant.

Buffer :

Phosphate buffered saline.

Preservative Stabilisers:

0.09% Sodium azide (NaN₃).

Carrier Free:

Yes.

Immunogen:

Concanavilin A activated Canine T cells.

Approx. Protein Concentrations:

IgG concentration 1.0 mg/ml.

Fusion Partners:

Spleen cells from immunised DA rats were fused with cells of the rat Y3/Ag1.2.3.myeloma cell line.

Regulatory:

For research purposes only.

Guarantee:

12 months from date of despatch.

Application:

Flow cytometry, immunohistology - frozen.

Storage:

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Note:

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.