Rat anti Dog CD45:RPE
Products Name
Rat anti Dog CD45:RPE
Cat.No.
CDMA0047
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Overview
Description:
Rat anti dog CD45 antibody, clone YKIX716.13 recognizes canine CD45 also known as leukocyte common antigen lustered as Canine CD45 in the First Canine Leukocyte Antigen Workshop (CLAW). Clone YKIX 716.13: immunoprecipitates an antigen of ~180/200 kDa from Con-A blasts. CD45 is expressed on all leukocytes in canine peripheral blood. Rat anti dog CD45 antibody, clone YKIX716.13 reacts with CD45 on all outbred mongrels and beagles tested and may be against CD45RB isoform.
Product Form:
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized.
Reconstitution:
Reconstitute with 1ml distilled water.
Preparation:
Purified IgG prepared by affinity chromatography on protein G from tissue culture supernatant.
Buffer :
Phosphate buffered saline.
Preservative Stabilisers:
0.09% Sodium azide.
1% Bovine serum albumin.
5% Sucrose.
1% Bovine serum albumin.
5% Sucrose.
Immunogen:
Canine thymocytes.
Fusion Partners:
Spleen cells from immunised DA rats were fused with cells of the Y3/Ag1.2.3 rat myeloma cell line.
Excitation Max (nm):
RPE 488 nm laser: 496.
Emission Max (nm):
RPE 488 nm laser: 578.
Regulatory:
For research purposes only.
Guarantee:
12 months from date of despatch.
Application:
Flow cytometry: use 10 µl of the suggested working dilution to label 106 cells in 100 µl.
Storage:
Prior to reconstitution store at +4°C. Following reconstitution store at +4°C.
Note:
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Product Type:
Monoclonal antibody
Clone:
YKIX716.13
Isotype:
IgG2b
Specificity:
CD45
Target:
Dog
