Description:
Rat anti canine CD4/CD8 antibodies, clones YKIX302.9 and YCATE55.9 is a dual colour reagent recognising canine CD4 and CD8 cell surface antigens. CloneYKIX302.9 recognises canine CD4, which is expressed by lymphocyte subsets and canine neutrophils.
Clone YCATE55.9 recognises CD8α expressed by cytotoxic T cells.
Product Form:
Dual colour combination consisting of FITC conjugated and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilized.
Reconstitution:
Reconstitute with 1.0 ml distilled water.
Buffer :
Phosphate buffered saline.
Preservative Stabilisers:
0.09% Sodium azide.
1% Bovine serum albumin.
5% Sucrose.
Antibody Isotypes:
FITC reagent: IgG2a (RAT).
RPE reagent: IgG1 (RAT).
Excitation Max (nm):
FITC: 490.
RPE 488 nm laser: 496.
RPE 561nm laser: 546.
Emission Max (nm):
FITC: 525.
RPE 488 nm laser: 578.
RPE 561nm laser: 578.
Application:
Flow cytometry: use 10 µl of the suggested working dilution to label 106 cells in 100 µl.
Storage:
Prior to reconstitution store at +4°C. Following reconstitution store at +4°C.
Note:
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Product Type:
Dual color reagent
Clone:
YKIX302.9/YCATE55.9