Rat anti Dog CD4:RPE
Products Name
Rat anti Dog CD4:RPE
Cat.No.
CDMA0036
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Overview
Description:
Rat anti dog CD4 antibody, clone YKIX302.9, is a monoclonal antibody specific for the canine CD4 cell surface antigen. Clone YKIX302.9 was clustered at the first Canine Leukocyte Antigen Workshop (CLAW) along with clone CA13.1E4.
Rat anti dog CD4 antibody, clone YKIX302.9 partially depletes circulating T lymphocytes when administered in vivo, but alone is not sufficient to prolong allograft survival in a canine transplant model.
Uniquely amongst mammals, canine CD4 is expressed by neutrophils as well as by lymphocyte subsets.
Rat anti dog CD4 antibody, clone YKIX302.9 partially depletes circulating T lymphocytes when administered in vivo, but alone is not sufficient to prolong allograft survival in a canine transplant model.
Uniquely amongst mammals, canine CD4 is expressed by neutrophils as well as by lymphocyte subsets.
Product Form:
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized.
Reconstitution:
Reconstitute with 1.0 ml distilled water.
Preparation:
Purified IgG prepared by affinity chromatography on protein G from tissue culture supernatant.
Buffer :
Phosphate buffered saline.
Preservative Stabilisers:
0.09% Sodium azide.
1% Bovine serum albumin.
5% Sucrose.
1% Bovine serum albumin.
5% Sucrose.
Immunogen:
Canine concanavilin A activated T cell blasts.
Fusion Partners:
Spleen cells from immunised DA rats were fused with cells of the Y3/Ag1.2.3 rat myeloma cell line.
Excitation Max (nm):
RPE 488 nm laser: 496.
Emission Max (nm):
RPE 488 nm laser: 578.
Regulatory:
For research purposes only.
Guarantee:
12 months from date of despatch.
Application:
Flow cytometry: use 10 µl of the suggested working dilution to label 106 cells or 100 µl whole blood.
Storage:
Prior to reconstitution store at +4°C. Following reconstitution store at +4°C.
Note:
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Product Type:
Monoclonal antibody
Clone:
YKIX302.9
Isotype:
IgG2a
Specificity:
CD4
Target:
Dog
