Rat anti Dog CD5:FITC

Description:

Rat anti dog CD5 antibody, clone YKIX322.3 recognizes canine CD5, a 67 kDa cell surface type 1 transmembrane glycoprotein also known as lymphocyte antigen T1, Ly-1 or Leu-1. CD5 is expressed on the surface of T-cells and thymocytes, CD5 is also expressed by NK cells at low levels. Rat anti dog CD5, cloneYKIX322.3 was clustered as canine CD5 in the First Canine Leucocyte Antigen Workshop.

In a study of 73 cases of canine chronic lymphocytic leukemia (CLL) CD5 expression was absent on all cases of B-cell CLL as defined by CD21 expression and lack of CD3 or other T cell antigen expression. Rat anti dog CD5 serves as a useful marker for the discrimination of canine leukemias of differing origins

Product Form:

Purified IgG conjugated to FITC - liquid.

Preparation:

Purified IgG prepared by affinity chromatography on protein G from tissue culture supernatant.

Buffer :

Phosphate buffered saline.

Preservative Stabilisers:

0.09% Sodium azide.
1% Bovine serum albumin.

Immunogen:

Concanavilin A activated canine peripheral blood cells.

Approx. Protein Concentrations:

IgG concentration 0.1 mg/ml.

Fusion Partners:

Spleen cells from an immunised DA rat were fused with cells of the rat Y3/Ag1.2.3 myeloma cell line.

Excitation Max (nm):

FITC: 490.

Emission Max (nm):

FITC: 525.

Regulatory:

For research purposes only.

Guarantee:

12 months from date of despatch.

Application:

Flow cytometry: use 10 µl of the suggested working dilution to label 10⁶ cells or 100 µl whole blood.

Storage:

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Note:

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.