Overview

Description:

Rat anti dog CD8 antibody, clone YCATE55.9 was clustered as Canine CD8 in the First Canine Leukocyte Antigen Workshop. YCATE55.9 reacts with a rat cell line transfected with cDNA for canine CD8α and blocks MHC class I dependant T-cell responses in vitro and in vivo .

Rat anti dog CD8, clone YCATE55.9 has been shown to deplete circulating CD8+ T cells when administered to dogs in vivo. Reduced levels of circulating CD8+ T cells has been associated with decreased survival times for dogs with osteosarcoma.

Product Form:

Purified IgG - liquid.

Preparation:

Purified IgG prepared by affinity chromatography on protein G from tissue culture supernatant.

Buffer :

Phosphate buffered saline.

Preservative Stabilisers:

0.09% Sodium azide (NaN₃).

Carrier Free:

Yes.

Immunogen:

Canine CD8 alpha chimaeric human IgG1 Fc fusion protein.

Approx. Protein Concentrations:

IgG concentration 1.0 mg/ml.

Fusion Partners:

Spleen cells from immunised DA rat were fused with cells of the Y3/Ag1.2.3 rat myeloma cell line.

Regulatory:

For research purposes only.

Guarantee:

12 months from date of despatch.

Application:

Flow cytometry, immunofluorescence, immunohistology - frozen.

Storage:

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Note:

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

Product Type:

Monoclonal antibody

Clone:

YCATE55.9

Isotype:

IgG1

Specificity:

CD8

Target:

Dog

Rat anti Dog CD8

Overview

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