Rat anti Dog CD8:FITC
Products Name
Rat anti Dog CD8:FITC
Cat.No.
CDMA0063
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Overview
Description:
Rat anti dog CD8 antibody, clone YCATE55.9 was clustered as Canine CD8 in the First Canine Leukocyte Antigen Workshop. YCATE55.9 reacts with a rat cell line transfected with cDNA for canine CD8α and blocks MHC class I dependant T-cell responses in vitro and in vivo .
Rat anti dog CD8, clone YCATE55.9 has been shown to deplete circulating CD8+ T cells when administered to dogs in vivo. Reduced levels of circulating CD8+ T cells has been associated with decreased survival times for dogs with osteosarcoma.
Rat anti dog CD8, clone YCATE55.9 has been shown to deplete circulating CD8+ T cells when administered to dogs in vivo. Reduced levels of circulating CD8+ T cells has been associated with decreased survival times for dogs with osteosarcoma.
Product Type:
Monoclonal antibody
Clone:
YCATE55.9
Isotype:
IgG1
Specificity:
CD8
Target:
Dog
Product Form:
Purified IgG conjugated to FITC - liquid.
Preparation:
Purified IgG prepared by affinity chromatography on protein G from tissue culture supernatant.
Buffer :
Phosphate buffered saline.
Preservative Stabilisers:
0.09% Sodium azide.
1% Bovine serum albumin.
1% Bovine serum albumin.
Immunogen:
Canine CD8 alpha chimaeric human IgG1 Fc fusion protein.
Approx. Protein Concentrations:
IgG concentration 0.1 mg/ml.
Fusion Partners:
Spleen cells from immunised DA rat were fused with cells of the Y3/Ag1.2.3 rat myeloma cell line.
Excitation Max (nm):
FITC: 490.
Emission Max (nm):
FITC: 525.
Regulatory:
For research purposes only.
Guarantee:
12 months from date of despatch.
Application:
Flow cytometry: use 10 µl of the suggested working dilution to label 106 cells in 100 µl.
Storage:
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Note:
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
