Rat anti Dog MHC Class II Monomorphic:FITC
Products Name
Rat anti Dog MHC Class II Monomorphic:FITC
Cat.No.
CDMA0083
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Overview
Description:
Rat anti dog MHC Class II Monomorphic antibody, clone YKIX334.2 recognizes a monomorphic epitope on canine MHC Class II and was classified at the First Canine Leucocyte Antigen Workshop.The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In dogs, this is referred to as the dog leukocyte antigen (DLA) region. Rat anti dog MHC Class II immunoprecipitates an antigen of ~32/34 kDa and blocks the proliferation of MHC Class II dependent responses in vitro . In dogs, MHC Class II is expressed by all peripheral blood mononuclear cells.
Product Form:
Purified IgG conjugated to FITC - liquid.
Preparation:
Purified IgG prepared by affinity chromatography on protein G from tissue culture supernatant.
Buffer :
Phosphate buffered saline.
Preservative Stabilisers:
0.09% Sodium azide.
1% Bovine serum albumin.
1% Bovine serum albumin.
Immunogen:
Con A activated canine peripheral blood cells.
Approx. Protein Concentrations:
IgG concentration 0.1 mg/ml.
Fusion Partners:
Spleen cells from immunised DA rats were fused with cells of the rat Y3/Ag1.2.3 myeloma cell line.
Excitation Max (nm):
FITC: 490.
Emission Max (nm):
FITC: 525.
Regulatory:
For research purposes only.
Guarantee:
12 months from date of despatch.
Application:
Flow cytometry: use 10 µl of the suggested working dilution to label 106 cells in 100 µl.
Storage:
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Note:
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Product Type:
Monoclonal antibody
Clone:
YKIX334.2
Isotype:
IgG2a
Specificity:
MHC Class II Monomorphic
Target:
Dog
