Rat Anti-ETA IgM Antibody ELISA

Product Overview:

The rat anti-ETA IgM antibody ELISA kit is designed for the determination of antibodies (IgM) against the Endothelin receptor subtype A in serum and plasma.

Product Description:

Endothelins (ET) are 21-amino acid vasoconstricting peptides produced primarily in the endothelium having a key role in vascular homeostasis. It mediates the effects through G-Protein-coupled receptors, the Endothelin receptors. There are two key receptor types, ETA and ETB. ETA receptors are found in the smooth muscle tissue of blood vessels where they increase vasoconstriction by ET-1. The Rat Anti Endothelin Receptor A IgM Antibody ELISA Assay kit is designed for the determination of antibodies (IgM) against the Endothelin receptor subtype A in serum and plasma.

Sensitivity:

Cut-off

Dynamic Range:

1.25 – 40 U/ml

Incubation Time:

3.5 hours

Sample Type:

Plasma, serum

Sample Size:

100 µl

Specimen Collection and Handling:

Dilute the samples with diluent using 1:100 dilution (eg. 5 µl serum or plasma + 495 µl diluent). If samples generate values outside the standard curve, the dilution factor may be quite varied. Store the undiluted samples at room temperature for 48 hours, 2-8°C 4- days, and long-term storage for up to 12 months at –20 °C. Avoid repeated freeze-thaw cycles.