Rat anti Human CD49f

Description:

Rat anti human CD49f antibody, clone NKI-GoH3 recognizes CD49f, also known as the VLA-6 alpha chain. CD49f is a 1107 amino acid ~120 kDa cell surface glycoprotein that forms distinct complexes with CD29 (VLA beta-chain), resulting in the VLA-6 (alpha-6 beta-1) complex, expressed on human platelets, or with the beta-4 integrin resulting in the alpha-6 beta-4 complex expressed on various human epithelial cells.

Rat anti human CD49f antibody, clone NKI-GoH3 reacts with platelets, megakaryocytes, T lymphocytes and common acute lymphoblastic leukemia cells (alpha-6 beta-1). In immunohistology the monoclonal antibody reacts with epithelial cells of a variety of tissues, peripheral nerves, microvascular endothelial cells, placenta cyto- and syncytotrophoblasts.VLA-6 is an important mediator of cell binding to laminin.

Rat anti human CD49f antibody, clone NKI-GoH3 blocks the binding of cells to the E8 fragment of laminin.

Product Form:

Purified IgG - liquid.

Preparation:

Purified IgG prepared by affinity chromatography on protein G from tissue culture supernatant.

Buffer :

Phosphate buffered saline.

Preservative Stabilisers:

<0.1% Sodium azide (NaN₃).

Carrier Free:

Yes.

Immunogen:

BALB/c mouse mammary tumor cells.

Approx. Protein Concentrations:

IgG concentration 1.0 mg/ml.

Fusion Partners:

Spleen cells from immunized Sprague-Dawley rats were fused with cells of the SP2/0 mouse myeloma cell line.

Regulatory:

For research purposes only.

Guarantee:

12 months from date of despatch.

Application:

Flow cytometry, immunofluorescence, immunohistology - frozen, immunoprecipitation.

Storage:

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Note:

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.