Description:
Rat anti MBP antibody, clone 12 recognizes myelin basic protein from a wide range of species. Rat anti MBP antibody, clone 12 reacts weakly with peptides ending in the Phe 91 where the 91-92 Phe-Phe bond is broken. Synthetic peptide 82-99 reacts very well with Rat anti MBP antibody, clone 12, as does intact MBP. Further epitope analysis indicates binding to a region defined by amino acids 82-87 (DENPVV).
Rat anti MBP antibody, clone 12 has been reported as being suitable for use in western blotting.
Product Form:
Tissue culture supernatant - liquid.
Preservative Stabilisers:
0.1% Sodium azide.
Fusion Partners:
Spleen cells from an immunised outbred rat were fused with cells of the mouse NS0 myeloma cell line.
Regulatory:
For research purposes only.
Guarantee:
12 months from date of despatch.
Application:
ELISA, immunofluorescence, radioimmunoassays, western blotting.
Storage:
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Note:
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Product Type:
Monoclonal antibody
Species Cross-Reactivity:
Mouse, rabbit, mammals, rat, guinea pig, sheep, human, chicken, pig