Rat anti Mouse CD11b:Biotin

Description:

Rat anti mouse CD11b antibody, clone M1/70.15 recognizes the murine CD11b cell surface antigen also known as the alpha M integrin chain or MAC-1, a differentiation antigen expressed by granulocytes, monocytes, NK cells and tissue macrophages.
The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages.

Rat anti mouse CD11b antibody, clone M1/70.15 has been reported to block iC3b binding to its receptor.
Rat anti mouse CD11b antibody, clone M1/70.15 has been reported to as being suitable for use on PLP fixed paraffin embedded tissue but has not been tested for use on formalin fixed tissue.
This product is routinely tested in flow cytometry on mouse peritoneal macrophages.

Product Form:

Purified IgG conjugated to biotin - liquid.

Preparation:

Purified IgG prepared by affinity chromatography on protein G from tissue culture supernatant.

Buffer :

Phosphate buffered saline.

Preservative Stabilisers:

0.09% Sodium azide.
1% Bovine serum albumin.

Immunogen:

T cell enriched splenocytes from B10 mice.

Approx. Protein Concentrations:

IgG concentration 0.1 mg/ml.

Fusion Partners:

Spleen cells from immunised DA rats were fused with cells of the NS1/1.Ag4.1 mouse myeloma cell line.

Regulatory:

For research purposes only.

Guarantee:

12 months from date of despatch.

Application:

Flow cytometry.

Storage:

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Note:

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.