Rat GIP ELISA Assay

Product Overview:

This rat GIP ELISA assay is used for quantitative determination of rat GIP (1-42) active form in plasma and culture medium supernatant. The kit is characterized by its sensitive quantification and high specificity. In addition, it has no influence by other components in samples. GIP (1-42) standard is highly purified synthetic product.

Assay Principle:

This rat GIP (Active) ELISA for determination of rat GIP (1-42) active form is based on a sandwich enzyme immunoassay. To the wells of plate coated with highly purified mouse monoclonal antibody against rat GIP (1-42) active form, standards or samples are added for the 1st step immunoreaction. After the 1st step incubation and plate washing, HRP labeled antibody solution against rat GIP (1-42) is added as the 2nd step to form antibody – antigen – labeled antibody complex on the surface of the wells. After the 2nd step incubation and rinsing out excess labeled antibody, Finally, HRP enzyme activity is determined by 3,3’,5,5’-Tetramethylbenzidine (TMB) and the concentration of rat GIP (1-42) active form is calculated.

Specificity/Target:

This assay kit has high specificity to rat GIP (1-42) active form and shows no cross reactivity to rat GIP (3-42) inactive form, glucagons, rat GLP-2, GLP-1 (7-36) NH2 and GLP-1 (9-36) NH2.

Dynamic Range:

3.9 – 250 pg/ml

Incubation Time:

3.5 hours

Sample Type:

Rat plasma, culture medium supernatant

Sample Size:

50 µl