Rat GLP-2 ELISA Assay

Product Overview:

Rat GLP-2 ELISA assay kit is used for quantitative determination of rat GLP-2 in serum or plasma samples. The kit is characterized for sensitive quantification, high specificity and no influences with other components in samples. Rat GLP-2 standard is highly purified synthetic product.

Assay Principle:

This rat GLP-2 ELISA for determination of rat GLP-2 in serum or plasma samples is based on a competitive enzyme immunoassay using combination of highly specific antibody to rat GLP-2 and biotin-avidin affinity system. The 96-wells plate is coated with goat anti rabbit IgG antibody. Rat GLP-2 standard or samples, labeled antigen and anti-rat GLP-2 polyclonal antibody are added to the wells for competitive immunoreaction. After incubation and plate washing, HRP labeled streptoavidin (SA-HRP) are added to form HRP labeled streptoavidin-biotinylated rat GLP-2-antibody complex on the surface of the wells. Finally, HRP enzyme activity is determined by　o-Phenylenediamine dihydrochloride (OPD) and the concentration of rat GLP-2 is calculated.

Dynamic Range:

0.137 – 100 ng/ml

Incubation Time:

20 hours

Sample Type:

Rat serum, plasma

Sample Size:

25 µl