Rat IL-4 ELISA Assay

Product Overview:

Rat IL-4 ELISA assay kit is used as an analytical tool for quantitative determination of Rat Interleukin 4, IL-4 in serum, plasma and cell culture supernatant.

Product Description:

The Rat IL-4 ELISA Assay Kit is used for assessing the specific biomarker in samples analytes which may be serum, plasma and cell culture supernatant as validated with the kit. The kit employs a sandwich ELISA technique which leads to a higher specificity and increased sensitivity compared to conventional competitive ELISA kits which employ only one antibody. Double antibodies are used in this kit.

Assay Principle:

The method employs sandwich ELISA technique. Monoclonal antibodies are pre-coated onto microwells. Samples and standards are pipetted into microwells and Rat Interleukin 4, IL-4 present in the sample are bound by the antibodies. Biotin labeled antibody is added and followed by Streptavidin-HRP is pipetted and incubated to form a complex. After washing microwells in order to remove any non-specific binding, the substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Rat Interleukin 4, IL-4 in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.

Sensitivity:

4.46 ng/l

Dynamic Range:

10 – 2000 ng/l

Incubation Time:

1 hour 10 minutes

Sample Type:

Serum, plasma, cell culture supernatant

Sample Size:

40 µl