Rat IL-6 ELISA Assay
Products Name
Rat IL-6 ELISA Assay
Cat.No.
IVEK0319
Size
Qty
Add to Cart
Inquiry
Overview
Product Overview:
The rat IL-6 ELISA assay (enzyme-linked immunoassay kit) is intended for the quantitative determination of rat interleukin 6 (IL-6) concentrations in cell culture supernates, serum, and plasma.
Assay Principle:
The rat interleukin 6 (IL-6) ELISA employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Interleukin 6 (IL-6) has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Interleukin 6 (IL-6) present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for Interleukin 6 (IL-6 ) is added to the wells and binds to the combination of capture antibody-Rat interleukin 6 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of Interleukin 6 (IL-6) present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven Interleukin 6 (IL-6) standard dilutions and Interleukin 6 (IL-6) sample concentration determined.
Sensitivity:
15 pg/ml
Dynamic Range:
62.5 – 2000 pg/ml
Incubation Time:
3 hours
Sample Type:
Serum, plasma, cell culture
Sample Size:
100 µl
Specimen Collection and Handling:
Cell culture supernates – remove particulates by centrifugation.
Serum – use a serum separator tube (SST) and allow samples to clot for 30 minutes before centrifugation for 15 minutes at approximately 1000 x g. Remove serum, avoid hemolysis and high blood lipid samples.
Plasma – recommended EDTA as an anticoagulant in plasma. Centrifuge for 15 minutes at 1000 x g within 30 minutes of collection.
Assay immediately or aliquot and store samples at -20°C. Avoid repeated freeze-thaw cycles.
Dilute samples at the appropriate multiple (recommended to do pre-test to determine the dilution factor).
Serum – use a serum separator tube (SST) and allow samples to clot for 30 minutes before centrifugation for 15 minutes at approximately 1000 x g. Remove serum, avoid hemolysis and high blood lipid samples.
Plasma – recommended EDTA as an anticoagulant in plasma. Centrifuge for 15 minutes at 1000 x g within 30 minutes of collection.
Assay immediately or aliquot and store samples at -20°C. Avoid repeated freeze-thaw cycles.
Dilute samples at the appropriate multiple (recommended to do pre-test to determine the dilution factor).
