Rat tPA Total ELISA Assay

Product Overview:

Rat tissue-type plasminogen activator (tPA) total antigen ELISA assay kit is for the quantitative determination of total tPA in rat biological fluids by a ELISA assay (enzyme-linked immunosorbent assay).

Assay Principle:

This rat tissue-type plasminogen activator (tPA) total antigen ELISA (enzyme-linked immunosorbent assay) is for the quantitative analysis of tPa levels in biological fluid. This test kit operates on the basis of sandwich ELISA where free, latent and complexed tPa is quantified with the use of an HRP labeled secondary antibody. The various forms of tPA present in the standard or unknown is captured by the tPA capture antibody coated on the well. A primary antibody specific for tPA is then added to each well followed by the HRP conjugated secondary antibody. The bound conjugated secondary antibody is detected by the addition of substrate, which generates an optimal color after 10 minutes. Quantitative test results may be obtained by the measure and comparison of the sample and standard absorbance readings when read with a microplate reader at 450 nm.

Sensitivity:

0.1 ng/ml

Dynamic Range:

0.1 – 50 ng/ml

Incubation Time:

2 hours

Sample Type:

Biological fluids

Sample Size:

100 µl