Recombinant Bovine Insulin-Like Growth Factor I

Description:

Recombinant bovine insulin-like growth factor I (IGF-I) is a recombinant protein corresponding to aa 50-119 of native bovine IGF-I.

IGF-I is a secreted mitogenic polypeptide and member of the insulin gene family, produced primarily by the liver, which effects a wide range of cells and plays a key role in cell proliferation and growth, inhibition of apoptosis, and is linked with tumor cell growth.

IGF-I signals by binding to the type 1 insulin-like growth factor receptor (IGF-IR) and also to the insulin receptor (IR), resulting in the activation of the AKT signaling pathway. IGF-I production is stimulated by growth hormone (GH) and can be hindered by GH insensitivity, deficiency in GH receptors or a malfunction in the post GH receptor signalling pathway.

Product Form:

Purified recombinant protein - lyophilized.

Reconstitution:

Reconstitute with 20 μl sterile PBS containing 0.1% carrier protein.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial.

Preparation:

Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography.

Buffer :

Phosphate buffered saline.

Preservative Stabilisers:

10% Trehalose.

Purity:

>95% by SDS PAGE analysis.

Protein Molecular Weight:

7.6 kDa.

Regulatory:

For research purposes only.

Guarantee:

12 months from date of despatch.

Application:

ELISA, western blotting.

Storage:

Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

Note:

Storage in frost-free freezers is not recommended. This product should be stored undiluted.