Recombinant Bovine Interferon Gamma

Description:

Recombinant bovine interferon gamma, (IFNγ), known previously as macrophage activating factor, belongs to the type II interferon family and is produced by T lymphocytes and NK cells.

Native bovine interferon gamma is a 143 amino acid cytokine with potent activating, anti-viral and anti-proliferative properties. It is produced as a pro-peptide with an additional 23 amino acid N-terminal signal peptide sequence having a molecular weight of ~20 kDa.

IFNγ is predominantly secreted by activated T lymphocytes in response to specific mitogens as a result of infection and has numerous immunological regulatory functions. Properties include macrophage activation, upregulation of MHC class I and II molecules on various cells and induction of cytotoxicity.

Product Form:

Purified recombinant protein - lyophilized.

Reconstitution:

Reconstitute with 100 μl sterile PBS containing 0.1% carrier protein.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial.

Preparation:

Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography.

Buffer :

Phosphate buffered saline.

Preservative Stabilisers:

10% Trehalose.

Purity:

>95% by SDS PAGE analysis.

Protein Molecular Weight:

16.9 kDa.

Regulatory:

For research purposes only.

Guarantee:

12 months from date of despatch.

Application:

ELISA, functional assays.

Storage:

Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

Note:

Storage in frost-free freezers is not recommended. This product should be stored undiluted.