Recombinant Bovine Interleukin-6

Description:

Recombinant bovine interleukin-6 (IL-6) is a recombinant protein corresponding to aa 30-208 of native bovine IL-6.

Bovine interleukin-6 is a multifunctional cytokine secreted by a range of cell types, in particular T lymphocytes and macrophages. IL-6 has a range of activities and includes the differentiation of B cells into Ig producing cells, differentiation of lymphocytes and of monocytes. IL-6 functions in both a pro-inflammatory role where it is an important inducer of the accute phase response and also as an anti-inflamatory myokine.

Interleukin-6 induces myeloma growth and is an essential cytokine required for successful hybridoma growth.

Product Form:

Purified recombinant protein - lyophilized.

Reconstitution:

Reconstitute with 20 μl sterile PBS containing 0.1% carrier protein.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial.

Preparation:

Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography.

Buffer :

Phosphate buffered saline.

Preservative Stabilisers:

10% Trehalose.

Purity:

>95% by SDS PAGE analysis.

Protein Molecular Weight:

20.7 kDa.

Regulatory:

For research purposes only.

Guarantee:

12 months from date of despatch.

Application:

ELISA, functional assays, western blotting.

Storage:

Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

Note:

Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.