Recombinant Dog Interferon Gamma

Description:

Recombinant dog interferon gamma, (IFNγ), known previously as macrophage activating factor, belongs to the type II interferon family and is produced by T lymphocytes and NK cells.

Native dog interferon gamma is a 166 amino acid cytokine with potent activating, anti-viral and anti-proliferative properties.

IFNγ is predominantly secreted by activated T lymphocytes in response to specific mitogens as a result of infection and has numerous immunological regulatory functions. Properties include macrophage and NK cell activation, upregulation of MHC class I and II molecules on various cells and induction of cytotoxicity.

Product Form:

Purified recombinant protein - lyophilized.

Reconstitution:

Reconstitute with 20 μl sterile PBS containing 0.1% carrier protein.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. BioVenic recommend that the vial is gently mixed after reconstitution.

Preparation:

Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography.

Buffer :

Phosphate buffered saline.

Preservative Stabilisers:

10% Trehalose.

Purity:

> 95% by SDS PAGE analysis.

Protein Molecular Weight:

16.7 kDa.

Regulatory:

For research purposes only.

Guarantee:

12 months from date of despatch.

Application:

ELISA, western blotting.

Storage:

Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.