Recombinant Dog TNF Alpha

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Recombinant Dog TNF Alpha
Products Name
Recombinant Dog TNF Alpha
Cat.No.
CC0024
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Overview

Description:
Canine tumor necrosis factor alpha (TNF alpha) is produced by a range of cells, including macrophages and monocytes and is an important mediator of the canine inflammatory response. Recombinant dog TNF alpha has an ED50 of 10 pg/ml, determined by measuring the cytolytic effect of recombinant canine TNF alpha on murine WEHI-13VAR-164 cells in the presence of actinomycin D.
Product Form:
Purified recombinant protein - lyophilized.
Reconstitution:
Reconstitute with 0.5 ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. BioVenic recommend that the vial is gently mixed after reconstitution.
Preparation:
Recombinant protein expressed in Pichia pastoris.
Buffer :
Phosphate buffered saline.
Preservative Stabilisers:
None present.
Purity:
> 95% by SDS PAGE analysis.
Protein Molecular Weight:
17.3 kDa.
Endotoxin Level:
< 1.1 EU/ug.
Regulatory:
For research purposes only.
Guarantee:
12 months from date of despatch.
Application:
Functional assays.
Storage:
Prior to reconstitution store at +4°C.
Following reconstitution store at -70°C.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Product Type:
Recombinant protein
Specificity:
TNF Alpha
Target:
Dog

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