Recombinant Dog TNF Alpha

Description:

Recombinant dog tumor necrosis factor alpha (TNF alpha), also known as tumor necrosis factor ligand superfamily member 2 (TNFSF2), is a recombinant protein corresponding to a 157 amino acid sequence of native canine TNF alpha. This recombinant protein is produced in yeast cells and is naturally folded and post translationally modified.

Canine TNF alpha is produced by a range of cells, including macrophages, monocytes, T and B lymphocytes and is an important mediator of the canine inflammatory response.

This product may be used as a standard in immunoassays.

Product Form:

Purified recombinant protein - lyophilized.

Reconstitution:

Reconstitute with 20 μl sterile PBS containing 0.1% carrier protein.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. BioVenic recommend that the vial is gently mixed after reconstitution.

Preparation:

Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography.

Buffer :

Phosphate buffered saline.

Preservative Stabilisers:

10% Trehalose.

Purity:

> 95% by SDS PAGE analysis.

Protein Molecular Weight:

16.9 kDa.

Regulatory:

For research purposes only.

Guarantee:

12 months from date of despatch.

Application:

ELISA, western blotting.

Storage:

Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.