Rodent beta Amyloid 1-40 ELISA

Product Overview:

The rodent beta amyloid 1-40 ELISA is intended for the quantification of beta-amyloid (1 – 40) in brain extract, cell culture supernatants, serum and plasma.

Assay Principle:

This ELISA kit is capable for measuring mouse and rat beta-Amyloid (1 – 40), it does not detect p3 peptide, which is cleaved by alpha or gamma secretase. This product is also useful for measuring mouse and rat derived beta-Amyloid in brain extract, cell culture supernatant and blood sample (serum or plasma) of mouse or rat. This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for beta-Amyloid (35 – 40) has been pre-coated onto a microtiter plate. Standards or samples are pipetted into the wells, and any beta-Amyloid (1 – 40) present is bound by the immobilized antibody. After washing away any unbound substances, a HRP-conjugated antibody specific for mouse/rat beta-Amyloid (1 – 16) is added to each well and incubate. After incubation, the capture antibody-antigen complex is developed and immobilized to the pre-coated wells. After washing away any unbound substances, a substrate solution (TMB) is added to the wells and color develops in proportion to the amount of beta-Amyloid (1 – 40) bound in the initial step. The color development is stopped by the addition of acid and the intensity of the color is measured at a wavelength of 450nm. The concentration of beta-Amyloid (1 – 40) in the sample is then determined by comparing the O.D of samples to the standard curve. The concentration of antigen is directly proportional to the optical density measured in the wells.

Sensitivity:

0.28 pg/ml

Dynamic Range:

1.56-100 pg/ml

Incubation Time:

3 hours

Sample Type:

Brain extract, serum, plasma, cell culture supernatants

Sample Size:

100 µl