Rodent HMGB1 ELISA Assay

Product Overview:

Mouse/rat HMGB1 ELISA assay kit is intended for the quantification of mouse/rat HMGB1 in mouse/rat plasma, cell culture supernatants and cell/tissue lysate samples.

Product Description:

Multifunctional redox sensitive protein with various roles in different cellular compartments. In the nucleus is one of the major chromatin-associated non-histone proteins and acts as a DNA chaperone involved in replication, transcription, chromatin remodeling, V(D)J recombination, DNA repair and genome stability. Proposed to be a universal biosensor for nucleic acids. Promotes host inflammatory response to sterile and infectious signals and is involved in the coordination and integration of innate and adaptive immune responses. In the cytoplasm functions as sensor and/or chaperone for immunogenic nucleic acids implicating the activation of TLR9-mediated immune responses, and mediates autophagy. Acts as danger associated molecular pattern (DAMP) molecule that amplifies immune responses during tissue injury. Released to the extracellular environment can bind DNA, nucleosomes, IL-1 beta, CXCL12, AGER isoform 2/sRAGE, lipopolysaccharide (LPS) and lipoteichoic acid (LTA), and activates cells through engagement of multiple surface receptors. In the extracellular compartment fully reduced HMGB1 (released by necrosis) acts as a chemokine, disulfide HMGB1 (actively secreted) as a cytokine, and sulfonyl HMGB1 (released from apoptotic cells) promotes immunological tolerance.

Sensitivity:

0.3 pg/ml

Dynamic Range:

0.3125-20 ng/ml

Incubation Time:

Overnight

Sample Type:

Plasma, cell culture supernatants, and cell/tissue lysate samples

Sample Size:

100 µl