Rodent IGFBP-2 ELISA Assay

Product Overview:

This rodent IGFBP-2 ELISA assay is suited for measuring IGFBP-2 in mouse and rat serum for scientific purposes.

Assay Principle:

The mouse/rat IGFBP-2 ELISA, is a so-called sandwich-assay. It utilizes two different specific high affinity polyclonal antibodies for this protein. The IGFBP-2 in the samples binds quantitatively to the immobilized antibody. In the following step, the biotinylated antibody in turn binds IGFBP-2. After washing, a streptavidin-peroxidase-enzyme conjugate will be added, which will bind highly specific to the biotin of the antibody. Subsequently, the peroxidase catalyzes an enzymatic reaction resulting in a blue coloration. The intensity of the blue color depends on the IGFBP-2 content of the sample. The reaction is stopped by the addition of stop solution and color intensity is quantified by measuring the absorption.

Sensitivity:

0.01 ng/ml

Incubation Time:

3 hours

Sample Type:

Serum from rat and mouse

Sample Size:

10 µl

Specimen Collection and Handling:

It is recommended to store samples as soon as possible at least at 4°C. For any long time storage the sample has to be kept frozen at -20°C. Samples were kept frozen and freeze-thaw cycles were applied. Up to 5 freeze-thaw cycles did not change the measured IGFBP-2 concentration significantly.