Swine IFN-gamma ELISA Assay

Product Overview:

The swine IFN-gamma ELISA assay (enzyme-linked immunoassay kit) is intended for the quantitative determination of swine interferon gamma (IFN-gamma) concentrations in cell culture supernates, serum, and plasma.

Assay Principle:

The swine interferon gamma (IFN-gamma) ELISA kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IFN-gamma has been pre-coated onto a microplate. Standard, control, or sample and the working solution of biotin-conjugate are pipetted into the wells. Following incubation and wash steps any IFN-gamma present is bound by the immobilized antibody and the detection antibody specific for IFN-γ is binds to the combination of capture antibody- IFN-γ in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of IFN-gamma present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IFN-γ standard dilutions and IFN-gamma sample concentration determined.

Sensitivity:

7 pg/ml

Dynamic Range:

15.625 ng/ml – 1000 ng/ml

Incubation Time:

3 hours

Sample Type:

Serum, plasma, cell culture

Sample Size:

100 µl