Swine IL-10 ELISA Assay

Product Overview:

Swine interleukin 10 (IL-10) ELISA assay kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of Swine Interleukin 10 (IL-10) concentrations in cell culture supernates, serum, and plasma.

Assay Principle:

Interleukin 10, also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 cytokine family. This family currently comprises IL-10, IL-19, IL-20, IL-22, IL-24, and IL-26/AK155. All IL-10 family members are secreted α-helical proteins. Porcine IL-10 is a secreted, possibly glycosylated, polypeptide with an 18 kDa molecular weight. Based on human studies, porcine IL-10 is likely to circulate as a nondisulfide-linked homodimer. Porcine IL-10 is synthesized as a 175 amino acid (aa) precursor with an 18 aa signal sequence and a 157 aa mature form. The mature segment has one potential N-linked glycosylation site plus four cysteines which form two intrachain disulfide bridges. Mature porcine IL-10 shows 71%, 70%, 76%, 75%, 77%, and 71% aa sequence identity to rat, mouse, human, guinea pig, canine, and cotton rat IL-10, respectively. Upon activation, mammalian cells known to secrete IL-10 include NK cells, cytotoxic CD8+ T cells secreting Th2-like cytokines, CD4+CD45RA- (memory) Th1 and Th2 cells, macrophages, monocytes, CD5+ and CD5- B cells, dendritic cells, hepatic stellate (Ito cells), keratinocytes, melanoma cells, mast cells, placental cytotrophoblasts, and fetal erythroblasts.

Sensitivity:

7 pg/ml

Dynamic Range:

15.625 ng/ml – 1000 ng/ml

Incubation Time:

3 hours

Sample Type:

Serum, plasma, cell culture

Sample Size:

100 µl