Swine IL-4 ELISA Assay

Product Overview:

The swine IL-4 ELISA assay is intended for the quantitative determination of Swine Interleukin 4 (IL-4) concentrations in cell culture supernates, serum, and plasma.

Product Description:

Interleukin 4 (IL-4), also known as B cell stimulatory factor 1, is a monomeric, approximately 13-18 kDa Th2 cytokine that shows pleiotropic effects during immune responses.It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four αhelix structure. Porcine IL-4 is synthesized with a 24 amino acid (aa) signal sequence. Mature porcine IL-4 shares 78%, 59%, 41%, and 41% aa sequence identity with bovine, human, mouse, and rat IL-4, respectively. Human IL-4 is active on porcine vascular endothelial cells. IL-4 exerts its effects through two receptor complexes. The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 Rα and the common γ chain (a shared subunit of the receptors for IL-4, -7, -9, -15, and -21). The type II receptor on nonhematopoietic cells consists of IL-4Rα and IL13Rα1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils. It promotes cell proliferation, survival, and immunoglobulin class switch to IgE in B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells. IL-4 plays a dominant role in the development of allergic inflammation and asthma.

Sensitivity:

7 pg/ml

Dynamic Range:

15.625 ng/ml – 1000 ng/ml

Incubation Time:

3.5 hours

Sample Type:

Serum, plasma, cell culture

Sample Size:

100 µl